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BMC Cancer 26 , 218 (2026)

For instance, in HeLa cells, glutathione redox potentials are more reducing in the mitochondrial matrix than in the cytoplasm (Couto et al., 2016), whereas the activity of glutathione transferases can differ between cellular compartments, including cytosol, mitochondria, and nucleus (Raza, 2011)

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The transsulfuration pathway is irreversible from cysteine and is reported to only occur in instances when levels of methionine are biologically dispensable but is also regulated by the availability of CSE and a biological need for cysteine (Homocysteine metabolism is reviewed in depth by Azzini et al, 2020)