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To identify and remove PCR duplicates, sequencing reads were deduplicated using CD-HIT 76 , which groups and removes duplicate reads that are identical within the first 150 nucleotides

With this study, we have found that people who smoke a pack a day develop an average of 150 extra mutations in their lungs every year, which explains why smokers have such a higher risk of developing lung cancer. Dr Ludmil Alexandrov First author from Los Alamos National Laboratory Until now, it has not been fully understood how smoking increases the risk of developing cancer in parts of the body that dont come into direct contact with smoke

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