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Of further importance, the health risk associated with smoking, whether passive or active, is exaggerated by obesity, and smoking and obesity are the leading causes of morbidity and mortality worldwide (11, 12)

For example, exposure was disproportionately higher among non-Hispanic blacks and those who had an income below the federal poverty level compared with non-Hispanic whites and those who had an income three or more times greater than the federal poverty level, respectively

Then, the purity and quantity of the isolated DNA were checked with a Nanodrop spectrophotometer ND-2000c (Thermo Scientific, USA) and subsequently preserved at -80 C