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However, for concentrations exceeding three g/dl, dilution with a 0.1 M phosphate buffer at pH 7.0 was necessary to obtain reliable results

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Mouse IgM (Invitrogen 88-50470-22, Pro-COL1A1(Abcam ab210579), NE (R&D DY4517), TSP1 (Abcam ab317551), COL4A1 (Novus Biologicals NBP2-75871), and COL6A1 (Novus Biologicals NBP3-42425) levels were quantified in BALF of mice using ELISA kits according to the manufacturers instructions