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Open 1 , e180756 (2018)

Under physiological conditions, the kinase PINK1 is stabilized on the outer membrane of depolarized mitochondria, subsequently recruiting and activating the E3 ubiquitin ligase Parkin to flag the damaged organelle for autophagic clearance

[DOI] [PMC free article] [PubMed] [Google Scholar] 13.A double- blind, cross-over, study of oral N-acetylcysteine in Sjgrens syndrome

The cell pellet was resuspended and lysed in lysis buffer (20 mM HEPES-NaOH pH 7.4, 200 mM NaCl, 1% Triton-X100 (v/v), 2 mM MgCl 2 , 0.5 mM TCEP) with protease inhibitors (1 mM PMSF, 1 M pepstatin, 4 M leupeptin, 0.3 M aprotinin) for 30 min at 4 C