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Weeks 1-4, Weeks 5-8 28 syringes 1 syringe per daily administration

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The remaining medial layer was cut into small pieces, placed in Dulbecco's modified Eagle's medium (DMEM) containing 4.5 g/L glucose, 10 mmol/L sodium pyruvate, and 20% FBS supplemented with 100 U/mL of penicillin and 100 g/mL of streptomycin, and incubated at 37 C in a humidified atmosphere containing 5% CO 2

Lancet 400(10366):18691881 Jastreboff AM, Kaplan LM, Fras JP, Wu Q, Du Y, Gurbuz S et al (2023) Retatrutide phase 2 obesity trial investigators