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The carryover puffing regime was 20 puffs, 2L/min flow rate, 2.4s puff length, 80mL puff volume, 30s inter-puff break and carryover was determined in triplicate (three separate cartridges)

Tracing back the generally accepted lethal dose to dubious self-experiments in the nineteenth century questioned the previously held standard stating that the lethal dose of nicotine for an adult was 60 mg or less (30-60 mg) as there had been several documented cases where humans survived higher doses