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GSMe is usually a much weaker inhibitor in the HEDS assay or in assays with GSSR substrates 11,14,17 , suggesting that the competition between GSH and GSMe in the roGFP2 measurements occurred at the 1st and not the 2nd glutathione interaction site

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( B ) Transgenic mouse treated with intranasal GHK-Cu

10.1074/jbc.M503075200 Veeravalli K, Boyd D, Iverson BL, Beckwith J, Georgiou G: Laboratory evolution of glutathione biosynthesis reveals natural compensatory pathways