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High abdominal pressure at 25, 30, 40, or 50 mmHg was maintained until sacrifice at 60 min (25 mmHg), 30 min (30 mmHg, 40 mmHg), or 15 min (50 mmHg)

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What you need on hand: The lyophilized peptide vial The correct solvent (the one named on the certificate of analysis) A fresh sterile syringe and needle for each draw Alcohol wipes and an alcohol-wiped work surface A labeled storage spot at 2 to 8C The full sequence, start to finish: Bring the sealed peptide vial and solvent to room temperature so condensation does not cloud the solution