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At the end, separated proteins were transferred to fluorescence-adapted polyvinylidene difluoride (PVDF) membrane (Immobilon FL-Membrane, Merck Millipore) using the following buffer (25 mM Tris-Base, 192 mM glycine, 0.0125% SDS and 20% methanol) at 400 mA for 60 min

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Generally, candidates have a BMI of 30 or higher, or a BMI of 27 or higher with weight-related health conditions such as high blood pressure, type 2 diabetes, or high cholesterol