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cDNA was amplified in MicroAmp Optical 96-well reaction plates with SYBR Green PCR Master Mix (Applied Biosystems) on an Applied Biosystems Prism 7000 sequence detection system

For metabolite clustering analysis, average linkage hierarchical clustering was performed in the Statistical Analysis module of MetaboAnalyst 6.0 using Euclidian distance as a similarity metric

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As such, supplementing FSH can improve deficiency, ultimately enhancing mitochondrial function and lowering oxidative stress levels in the body