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ROS and lipid peroxidation assay Day 12 cultured SCD erythroblasts under different treatments were incubated with 5 M 2-7-dichlorodihydrofluorescein diacetate (H2DCFDA) (#D6883, Sigma) for 30 min at 37 C, washed with PBS, and analyzed on a BD LSR-II flow cytometer for ROS level

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Its advisable to start with a lower dose and consult a healthcare provider

Vitamin B6, in the form of pyridoxal-5'-phosphate, is the cofactor for 5 enzymes in the -glutamyl cycle and trans-sulphuration pathway: cystathionine -synthase, cystathione -lyase, cytoplasmic and mitochondrial serine hydroxymethyltransferase and glycine decarboxylase in the mitochondria