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Activity Assays for Recombinant Cr GPDH Activity of the NAD-dependent glycerol-3-phosphate dehydrogenase domain of Cr GPDH was monitored in a continuous assay by following the absorbance at 340 nm ( 340 =6.2 mM 1 cm 1 ) using a Cary 3500 UV-Vis spectrophotometer (Agilent Technologies)

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If small particles remain after 5 minutes of gentle swirling, let the vial sit in the refrigerator for 15 to 20 minutes and check again