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Curr Nutr Rep 12(1):5664

cAMP T EPAC VV Fluorescence Resonance Energy Transfer (FRET) imaging Islets extracted from Pdx1 Cre-ERT -CAMPER mice, conditionally expressing the T EPAC VV cAMP biosensor in beta cells under the control of the Pdx1 promoter conjugated to a mutant oestrogen receptor sequence (63), were incubated in 1 M 4-hydroxytamoxifen for 24 hours to induce T EPAC VV expression, treated with simvastatin as above, washed, encased in Matrigel and imaged in 6 mM glucose KRBH buffer using a Zeiss LSM-780 inverted confocal laser-scanning microscope in a 20X objective at 37C

Reconstitution A: 5 mg vial + 2 mL BAC water (2.5 mg/mL) practical mid-range dilution where 1 mg = 40 units

By design, GLOW assigns one peptide to address each bottleneck