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Based on their pKa values (Supplementary Table 1) predicted by PROPKA 23,24 and the pH of our experimental assays (pH = 8), we modeled His442 in its protonated state in both monomers and the LLP as 1 and 2 charged in monomer I and II of apo- Pc ncAAAD, respectively (see Method for details)

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Good sources on smoking and prenatal development: Smoking, Pregnancy And Babies (CDC) Smoking During Pregnancy (March of Dimes) Tobacco and Pregnancy (ACOG) How Smoking Effects you and Your Baby During Pregnancy (Cleveland Clinic) 8 Dangers of Smoking while Pregnant (Healthline) Still need help to stop smoking while pregnant